1. To which country is the expedition, where they discover the deadly new species of spider?
From Quiz Are You Afraid of Spiders?
Answer:
Venezuela
Manley, the photographer, is the first to reference their location when he says he is late as he was stuck in Caracas, which is the capital of Venezuela.
It is then mentioned a few more times by various characters throughout the movie.
Dr James Atherton (Julian Sands) and his team are in the rainforest looking for new insect species when they come across some previously unseen spiders. They appear immune to the chloroform and are highly aggressive. The most fascinating thing for the scientists, however, is that they behave more like organised insects (ants/bees), having one fertile male, a queen and drones (soldiers).
In real life, the Alpha male spider is actually a species of Bird Eating Tarantula and the soldiers are Avondale spiders, a harmless species from New Zealand.